The ZIC1 Antibody (CAB17547) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications. It binds specifically to the ZIC1 protein, enabling accurate detection and analysis in a variety of cell types.ZIC1 plays a critical role in regulating gene expression and cell fate determination, making it a key player in developmental processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ZIC1 Antibody
SKU:
CAB17547
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ZIC, CRS6, BAIDCS, ZNF201, ZIC1
Positive Sample:
U-87MG, U-251MG, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Nucleoplasm, Nucleus.
Calculated MW:
48kDa
Observed MW:
48kDa
This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development. Aberrant expression of this gene is seen in medulloblastoma, a childhood brain tumor. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4, a related family member on chromosome 3. This gene encodes a transcription factor that can bind and transactivate the apolipoprotein E gene.
Purification Method
Affinity purification
Gene ID
7545
RRID
AB_2772964
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ZIC1 Rabbit pAb (CAB17547) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
