The HDAC6 Monoclonal Antibody (CAB3572) is a high-quality antibody developed for reliable detection and analysis of target proteins. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HDAC6 Monoclonal Antibody
SKU:
CAB3572
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0805
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:100 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HD6, JM21, CPBHM, PPP1R90, HDAC6
Positive Sample:
HeLa, Hep G2
Cellular Localization:
Axon Cytoplasm, Caveola, Centrosome, Ciliary Basal Body, Cytoplasm, Cytosol, Histone Deacetylase Complex, Multivesicular Body, Nucleoplasm, Nucleus, Perinuclear Region Of Cytoplasm.
Calculated MW:
131kDa
Observed MW:
160kDa
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
Purification Method
Affinity purification
Gene ID
10013
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HDAC6 Rabbit mAb (CAB3572) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HDAC6 Rabbit mAb (CAB3572) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
