![Anti-Human CD3 [UCHT-1] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214452/569375/anti-human-cd3-ucht-1-in-vivo-antibody-low-endotoxin__97011.1673496659.jpg?c=2?imbypass=on "Anti-Human CD3 [UCHT-1] In Vivo Antibody - Low Endotoxin")
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Human CD3 [UCHT-1] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0117 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | UCHT-1 |
| Protein: | CD3 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | T3, CD3-epsilon |
| Isotype: | Mouse IgG1 κ |
| Reactivity: | Human |
| Immunogen: | This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunized against human lymphocytes, with UCHT1 being one of the first successful fusions. |
| Applications: | Act, CyTOF®, FC, IF Staining, IHC FF, IP, PhenoCycler®, WB\nWorkshops III 471 |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | Act, CODEX®, CyTOF®, FC, IF Staining, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone UCHT-1 binds to an acidic region of CD3-epsilon, occluding this region from direct interaction with the T cell receptor. This antibody is considered a pan T-cell marker. Furthermore, this antibody can be used for the detection of T cell populations in peripheral blood, lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia’s. It reacts with the majority of peripheral blood T lymphocytes, a significant proportion of thymocytes, the majority of T cell chronic lymphocytic leukemia cells and approximately 70% of acute lymphoblastic leukaemia’s of T cell origin. |
| Antigen Distribution: | CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells. |
| Immunogen: | This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunized against human lymphocytes, with UCHT1 being one of the first successful fusions. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
| Technical Datasheet: | View |
| Protein: | CD3 |
| Function: | Antigen recognition, signal transduction, T cell activation |
| Ligand/Receptor: | Peptide antigen bound to MHC |
| Research Area: | Immunology |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
![Anti-Human CD3 [UCHT-1] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/214452/569375/anti-human-cd3-ucht-1-in-vivo-antibody-low-endotoxin__97011.1673496659.jpg?c=2 "Anti-Human CD3 [UCHT-1] In Vivo Antibody - Low Endotoxin")
