The Anti-Pan-Ubiquitin Remnant Motif K-GG Antibody (CAB20303) is a highly specific antibody designed for research involving the ubiquitin system, a key regulator of protein degradation and cell signaling. This antibody, raised in rabbits, specifically targets the K-GG motif present in ubiquitin remnants, allowing for precise detection and analysis in various biological samples.Ubiquitination is a post-translational modification crucial for protein turnover, DNA repair, and signal transduction pathways. Dysregulation of the ubiquitin system has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
The Anti-Pan-Ubiquitin Remnant Motif K-GG Antibody provides a valuable tool for studying ubiquitin-mediated processes and identifying potential therapeutic targets for these diseases.With its high specificity and sensitivity, this antibody is ideal for use in immunoprecipitation, Western blotting, and other molecular biology techniques. Researchers in the fields of cell biology, biochemistry, and drug discovery will find the Anti-Pan-Ubiquitin Remnant Motif K-GG Antibody indispensable for their studies on the ubiquitin system and its role in disease pathogenesis.
A synthetic peptide corresponding to a sequence containing ubiquitinated Motif (K-ε-GG)
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
HeLa,NIH/3T3,Mouse brain,C6,Rat heart,293T
Conjugate:
Unconjugated
Observed MW:
>10kDa/＞10kDa
Ubiquitination plays a key role in protein degradation and signal transduction. Characterization of ubiquitination sites is important for understanding the role of this modification in cellular processes and disease. Ubiquitination sites are usually identified by detection of Lys-?-Gly-Gly (K-?-GG)-remnant peptides, which are generated by tryptic digestion of proteomes. The di-glycine remnant left at sites of ubiquitination after trypsin digestion through cleavage of the C-terminal –RGG sequence on ubiquitin (K-ε-GG). This Ubiquitin K-ε-GG remnant motif antibody will recognize the di-glycine remnant independent of flanking amino acid sequence.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells using pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (CAB20303) at 1:1000 dilution. 293T cells were treated by MG132(5 μM) at 37℃ for 4 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
