Description
Bovine Cytokine Array (10 targets) (SARB0003)
The Bovine Cytokine Array 10 Targets (SARB0003) is a powerful tool for researchers studying cytokine expression in bovine samples. This array contains antibodies specific to 10 different cytokine targets, allowing for the simultaneous detection and quantification of multiple cytokines in a single experiment. The array is compatible with a variety of sample types, including serum, plasma, cell lysates, and tissue homogenates, making it versatile for a wide range of research applications. Researchers can easily analyze cytokine levels in bovine samples using this array, providing valuable insights into the immune response and inflammatory processes in cattle.
With its high sensitivity and specificity, the Bovine Cytokine Array 10 Targets is a valuable tool for studies in immunology, veterinary medicine, and livestock health. By comprehensively profiling cytokine expression patterns, researchers can gain a better understanding of the immune response in bovine species, paving the way for the development of new diagnostic tools and therapeutic strategies.
| Product Sku: | SARB0003 |
| Size: | 2, 4, or 8 |
| Species Detected: | Bovine |
| Number of Targets Detected: | 10 |
| Gene Symbols: | CCL4, CXCL10, CXCL9, IFNA1, IFNG, IL13, IL1A, IL21, IL36RN, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
IFN alpha
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-36 Ra (IL-1 F5)
| IL-13
|
IL-21
| IP-10 (CXCL10)
| MIG (CXCL9)
| MIP-1 beta (CCL4)
| TNF alpha
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Bovine Cytokine Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
