The CISH Antibody (CAB14527) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the CISH protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in immunology, cancer research, and beyond.CISH, also known as cytokine-inducible SH2-containing protein, plays a crucial role in regulating the immune response by modulating cytokine signaling and downstream pathways. Its involvement in immune system balance and inflammation makes it a key player in the development of therapies for conditions such as autoimmune diseases, inflammatory disorders, and cancer.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CISH Antibody
SKU:
CAB14527
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CIS, G18, SOCS, CIS-1, BACTS2, CISH
Positive Sample:
K-562, Mouse kidney, Rat kidney
Cellular Localization:
Cytosol, Plasma Membrane.
Calculated MW:
29kDa
Observed MW:
37kDa
The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1154
RRID
AB_2761403
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CISH Rabbit pAb (CAB14527) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of C6 cells using CISH Rabbit pAb (CAB14527) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
