The Dimethyl-Histone H3 (K4) Polyclonal Antibody (CAB2356) is a valuable tool for researchers studying epigenetic modifications involved in gene regulation. This antibody, produced in rabbits, is highly specific to dimethylated histone H3 at lysine 4 (H3K4me2) and is validated for use in various applications, including immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation (ChIP).Histone H3 methylation at lysine 4 is known to be associated with active gene transcription, making it a key epigenetic mark in the regulation of gene expression. The Dimethyl-Histone H3 (K4) Antibody enables researchers to specifically detect and study this modification in various cell types and tissues, providing valuable insights into the mechanisms underlying gene regulation and cellular functions.
By targeting dimethylated histone H3 at lysine 4, this antibody allows for the investigation of epigenetic changes that contribute to diseases such as cancer, neurodegenerative disorders, and developmental abnormalities. Understanding the role of H3K4me2 in these pathological conditions can lead to the development of novel therapeutic strategies targeting epigenetic pathways for disease treatment and prevention.
Product Name:
DiMethyl-Histone H3-K4 Rabbit Polyclonal Antibody
SKU:
CAB2356
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DiMethyl-Histone H3-K4 Rabbit pAb (CAB2356) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
