The EPHA4 Antibody (CAB8346) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity with EphA4 in human samples, making it ideal for Western blot applications. By targeting the EphA4 protein, this antibody enables precise detection and analysis of EphA4 expression in different cell types, making it an invaluable asset for investigations in neurobiology, development, and cancer research.EphA4 is a member of the Eph receptor tyrosine kinase family, known for its involvement in axon guidance, synaptic plasticity, and cell migration.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EPHA4 Antibody
SKU:
CAB8346
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EK8, SEK, HEK8, TYRO1, EPHA4
Positive Sample:
Mouse brain, Mouse lung, Rat brain
Cellular Localization:
Cell Junction, Cell Membrane, Cell Projection, Early Endosome, Single-Pass Type I Membrane Protein, Axon, Dendrite, Postsynaptic Cell Membrane, Postsynaptic Density, Synapse.
Calculated MW:
110kDa
Observed MW:
110kDa
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2043
RRID
AB_2769336
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EPHA4 Rabbit pAb (CAB8346) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunofluorescence analysis of C6 cells using EPHA4 Rabbit pAb (CAB8346) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
