GeniePlex Multiplex FAQs
Multiplex Assays
FAQs & Troubleshooting
Pre- and Post-experimental support for our GeniePlex Multiplex Assays!
GeniePlex Multiplex Protocol
Pre-Experimental Support
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FAQ
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Protocols
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Flow Cytometer Setup
| Question | Answer |
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Which species are suitable for GeniePlex Immunoassays? |
Human, Non-Human Primate, Canine, Mouse and Rat. |
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Which sample types are suitable for GeniePlex Immunoassays? |
Cell Culture Supernatant, Serum, Plasma, Cell/Tissue Lysate, Saliva, BALF, Pleural Fluid, Peritoneal Fluid, Synovial Fluid and Cerebrospinal Fluid. |
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What is the maximum number of targets you can multiplex in a single panel? |
Up to 24 targets in a single panel. |
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What Flow Cytometer can be used? |
Any Flow Cytometer equipped with either a single 488nm laser or dual 488nm and 633/640nm lasers. |
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Does it need a Plate Loader? |
No, this assay can also be run using FACS tubes. Process the assay as per protocol using the filter plate. After these wash steps, transfer the samples to test tubes and acquire on a Flow Cytometer. |
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Can I use a Luminex* to analyze GeniePlex assays? |
No. |
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Is there any other equipment needed? |
GeniePlex Filter Plate Washer NX (ACAM00001) and Plate Shaker. |
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Can I create my own multiplex panel? |
Yes - https://www.assaygenie.com/custom-multiplex-panels |
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If the assay/panel I want is not available, what should I do? |
Send an email to our development team info@assaygenie.com or send a message to LiveChat. |
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How will I analyze the data? |
We recommend using Soft Flow’s FCAP Array* V3 or Infinite. You can also use You can use FlowJo to obtain PE MFIs (median fluorescence intensity) of each bead set then use a software package (e.g. Prism or https://elisaanalysis.com/) that can perform nonlinear regression analysis to carry out curve fitting and concentration calculations. |
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What is a GeniePlex Filter Plate Washer? |
A filter plate washer (vacuum manifold) is required for the wash steps. GeniePlex Filter Plate Washer: https://www.assaygenie.com/genieplex-filter-plate-washer/ This filter plate washer is a once-off purchase, used to increase the sensitivity and specificity of GeniePlex assays. It can be reused when needed. |
Protocol Steps
| Step | Protocol |
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1. |
Prepare the filter plate template. Mark the standard, sample and blank wells. Standards and samples should be run in duplicates or triplicates. If the whole plate will not be used, seal the unused well with a plate seal. |
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2. |
Vortex working bead suspension for 15 seconds. |
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3. |
Add 45 µL of capture bead working suspension to each well. NOTE: Save the remaining capture bead working suspension and store at 2-8°C with light protection. It can be used for setting up acquisition parameters on the flow cytometer. |
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4. |
Remove buffer in the wells by using the “flow-through“ Filter Plate Washer connected to a vacuum source that has been adjusted according to the Filter Plate Washer Instructions. |
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5. |
Remove buffer in the wells by using the “flow-through“ Filter Plate Washer connected to a vacuum source that has been adjusted according to the Filter Plate Washer Instructions. |
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6. |
Add 30 µL of CCS, SPB or TL Assay Buffer to each sample well. NOTE: Cell culture supernatant samples can be run without diluting in Assay Buffer if very low levels (less than 20 pg/mL) of cytokines are expected. If it is the case, skip this step and add 45 µL of cell culture supernatant samples to each sample well in Step 7. |
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7. |
Add 15 µL of samples to each sample well. Add 45 µL of standards to each standard well. Cover the plate with a plate seal. |
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8. |
Incubate on the shaker (set at 700 rpm) for 60 min at room temperature. Protect from light by wrapping the filter plate in aluminum foil. |
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9. |
Remove the plate seal. Remove solutions in the wells by using the Filter Plate Washer connected to a vacuum source. |
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10. |
Remove solutions in the wells by using the Filter Plate Washer connected to a vacuum source. |
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11. |
Wash the wells three times with 100µL 1x Wash Buffer using the Filter Plate Washer. |
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12. |
Gently tap the plate bottom onto several layers of paper towels to remove residual buffer on the plate bottom after the last wash. |
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13. |
Add 25µL of biotinylated antibody working solution to each well. Cover the plate with a plate seal. |
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14. |
Incubate on the shaker (set at 700 rpm) for 30 min at room temperature. Protect from light by wrapping the filter plate in aluminum foil. |
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15. |
Remove the plate seal. Remove solutions in the wells by using the Filter Plate Washer. Wash the wells three times with 100 µL 1x Wash Buffer using the Filter Plate Washer. |
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16. |
Gently tap the plate bottom onto several layers of paper towels to remove residual buffer on the plate bottom after the last wash. |
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17. |
Add 25µL of streptavidin-PE working solution to each well. Cover the plate with a plate seal. |
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18. |
Incubate on the shaker (set at 700 rpm) for 20 min at room temperature. Protect from light by wrapping the filter plate in aluminum foil. |
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19. |
Remove the plate seal. Remove solutions in the wells by using the Filter Plate Washer. Wash the wells twice with 100 µL 1x Wash Buffer. |
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20. |
Gently tap the plate bottom onto several layers of paper towels to remove residual solution. Add 150µL to 300µL of 1x Reading Buffer to each well depending on the sample loading mechanism of a flow cytometer to re-suspend the beads. Cover the plate with a plate seal. |
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21. |
Place the plate on the microtiter shaker and shake for 30 seconds at 700 rpm. NOTE: If the flow cytometer has no 96-well plate loader and more than 200 µL of 1x Reading Buffer is needed to re-suspend the beads, do not shake the plate. Re-suspend the beads in each well by pipetting up and down 6–8 times with a P200 pipette then transfer to a test tube for acquisition. Remove the plate seal. Read on a flow cytometer. |
Post-Experimental Support
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Low level count |
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Low assay signal or sensitivity |
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High Background |
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Low Precision |
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Clogged filter plate |
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Customer FAQs
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Analytes
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Components
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Competitors
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Data Analysis
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Protocol
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Flow Cytometer
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QC
| Question | Answer |
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Do you ever come across issues when creating custom multiplex panels? |
When we quote you for a custom panel, even if we do not see an issue to multiplex these analytes as a single panel, we won’t know for sure until we develop this custom panel after receiving a PO from you. If we find issues to multiplex them as a single panel, we will offer the corresponding numbers of extra basic kit(s) and diluent kit(s) at no charge to run as more than 1 panel. |
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Is synovial fluid a compatible sample type? |
Synovial fluid is compatible. It should be free of cells. If there is a possibility of cells in synovial fluid samples, centrifuge the samples at 300xg for 10 mins at 2-8 C and collect the supernatant fractions for GeniePlex assays. |
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I'm worried about the low expression levels of my cytokines. Is there a recommended step to detect lower levels of cytokines? |
If you are worried about lowly expressed cytokines please refer to section 8.2 of the manual. NOTE: If very low levels (less than 10 pg/mL) of cytokines are expected in the samples, we recommend adding 1 or even 2 more standard points, e.g. Standard 8 (1/2187) and Standard 9 (1/6561) in the assay for better quantitation at low concentrations. Standard 1 may be omitted when assaying those samples. |
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Can TGF-beta be multiplexed with other targets? |
TGF beta needs to be run on a separate panel due to sample pre-treatment. |
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What is the pre-treatment process for TGF beta? |
Samples should be pretreated with HCl then neutralized to activate latent TGFβ1 to the immunoreactive form for accurate measurement. Do not pretreat the TGFβ1 Standard. Add 20 µL of 1N HCl to 100 µL of sample. Incubate for 10 minutes at room temperature after mixing. Neutralize with an addition of 20 µL of 1.2N NaOH/0.5M HEPES. The activated samples may need to be diluted with 1x TGFbeta Assay Buffer for the assay. The appropriate dilution factor should be determined by the investigator according to the sample type and experimental conditions. Because of the sample pretreatment, the TGFβ1 assay cannot be multiplexed with any other GeniePlex assays. |
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How do you differentiate one analyte from another if the variation is too large? i.e. if one protein is highly expressed versus another protein which is lowly expressed |
If you can let us know the sample type and the analytes, we will be able to offer a suggestion of the panel(s). For example, if it is human CRP in serum or plasma, pre-diluting samples is required because CRP levels in normal serum/plasma samples are very high. Because of the sample pre-dilution requirement, CRP cannot be run with other cytokines such as IL-1beta, IL-6 etc. in a panel. In such a case, 2 panels will be quoted: Panel 1: CRP; Panel 2: IL-1beta, IL-6 etc. |
| Question | Answer |
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Can the plates be reused? |
The unused wells can be used in another experiment. Please note, there is only one vial of lyophilized standard mix in each kit. If multiple runs are needed, corresponding numbers of extra vial(s) of standard mix need to be ordered. |
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Can the seals be reused? |
No. There are 4 seals in each kit. Please do not reuse them because of possible cross contamination. |
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Can you use an ELISA washer for plate washing rather than a filter plate washer? |
No. The ELISA plate washer does not work for GeniePlex assays since ELISAs normally coat wells with capture antibodies. GeniePlex beads are in suspension and require a filter plate for washing. |
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For this step "Use a filter plate washer connected to a vacuum source to remove the solution in the wells." What is the pressure of the vacuum source? |
Please refer to the Filter Plate Washer manual (https://www.assaygenie.com/genieplex-filter-plate-washer/) for installation instruction. "An economy vacuum pump (e.g. Barnant Model 400-1901; Wikita Model ZK-26 - Oil free diaphragm type vacuum pump pressure 0~0.08mpa, 1000ml; or equivalent) is recommended". "Step 5: Turn the vacuum ON then turn the vacuum OFF as soon as the liquid in the wells starts to go down. It should take 3-5 seconds to empty 100 μL of ddH2O from the wells with the remaining vacuum in the system after it is turned OFF. Vacuum pressure should be adjusted accordingly. Repeat Steps 2-5 as necessary for proper adjustments." |
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Can I use a central vacuum system? |
We do not recommend using a central vacuum system because it is usually very strong and not easy to turn on and off with a faucet, however we've had GeniePlex customers use this successfully. An electric vacuum pump with a power switch is recommended (please refer to the user manual of recommended models). Vacuum source for the filter plate washer. An economy vacuum pump (e.g. Barnant Model 400-1901; Wikita Model ZK-26 - Oil free diaphragm type vacuum pump pressure 0~0.08mpa, 1000ml; or equivalent) is recommended. |
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Does the standard stock solution contain all the antigens that are being analysed? |
Yes. Our antigen standards are premixed and lyophilized and consist of all the standards in a panel. It is one single premixed standard vial for all the standard premixed panels and the vast majority of custom panels. |
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Will unused wells be affected by repeated pumping? |
No. |
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Does the plate come as removable strips? Can I use the plate across several tests? |
It is a filter plate. No strips. If a whole plate will not be used, seal unused wells with a plate seal. |
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What is the shelf life of the kit? |
We do not ship any kit that has a shelf life of less than 6 months. Our kits usually have a shelf life of more than a year. We can accommodate longer term studies such as projects from CROs with a shelf life of 2 years which is the longest. There is an expiration date on each of our kits. |
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What is the purpose of the extra plate that comes with the Filter Plate Washer? |
This plate is a practice filter plate. This is to allow you to practice controlling the flow rate, e.g. testing/aspirating with distilled water over a three second period. |
| Question | Answer |
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GeniePlex vs Meso Scale Discovery (MSD) |
Sample Volume: GeniePlex 15uL vs 25uL for MSD Larger Panels: GeniePlex can comfort 1-24 vs 7-10 for MSD Custom Assay Development: In contrast to MSD, we primarily provide custom multiplex assays tailored to your needs. These custom assays are quick & easy to build and have a lead time of just 5-7 working days. Flexibility: GeniePlex uses any flow cytometer so no need for a dedicated Mesoscale instrument which is expensive. Cost: We are competitive with price per plate but also there are no quarterly fees as is the case with MSD for upkeep & maintenance. Local: No customs delays or expensive delivery charges. In addition, we have local tech support with a response time of <5 hour. 3 Training Sessions: Free Set-up, Optimisation & Data Analysis Support. Saturation: This can be an issue with MSD, not with GeniePlex |
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GeniePlex vs BD CBA (& iQueBeads) |
Sensitivity: Our assays are more sensitive than Homogenous bead-based assays because of washing steps (heterogeneous vs. homogeneous). When conducting drug screening using in vitro models such as a cell line, some markers can be extremely high after the addition of a drug candidate. With a washing step after cAb-Bead and antigen-binding to remove unbound antigens, false negatives can be avoided which is crucial for drug screening projects. Error & Time Reduction: GeniePlex beads and standards come pre-mixed in vials. iQueBeads & CBA beads come in separate vials so there is an increased risk of error & time required. Validation: All of our custom panels are fully validated and tested for cross-reactivity before they leave the lab. However, CBA's custom assays are 'off-the-shelf' so there is a risk of cross-reactivity. 3 Training Sessions: Free Set-up, Optimisation & Data Analysis Support. High Throughput: We can develop assays to be amenable to high-throughput and automated liquid handling systems, we use magnetic beads in a 384-well format. Sample volume required for Magnetic bead assays 6uL. |
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GeniePlex vs Luminex/BioPlex/ Milliplex |
Sample Volume: 15ul sample size vs 50uL for Luminex Flexibility: No need for a Luminex instrument (GeniePlex can be run on any flow cytometer) Custom: Custom panel development in 7 business days Cost: No minimum order quantity required for premade or custom panels 3 Training Sessions: Free Set-up, Optimisation & Data Analysis Support. |
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GeniePlex vs Legendplex |
Larger Panels: Quantify up to 24 analytes vs 14 analytes with LegendPlex 150 More Analytes Available: GeniePlex 470 vs Legenplex 312 Custom Panel Optimization: To maintain excellent assay performance, we don't force antibodies onto the same panel if they cannot be conveniently multiplexed with another analyte. For example, receptors and ligands or closely related proteins eg: CXCL1/GROalpha and CXCL2/GRObeta. Cross-Reactivity Testing: All GeniePlex Custom Multiplex Assays are fully tested for cross-reactivity before they leave the lab Short Lead Times: All panels in 10 days vs 6-8 weeks Cost: No minimum order requirement for custom panel development - LegendPlex typically requires a minimum of 5 plates for a custom panel order 3 Training Sessions: Free Set-up, Optimisation & Data Analysis Support. Filter Plate Washer: We put a lot of emphasis on the sensitivity and consistency of our assays. Using a filter plate washer results in faster and more consistent washing of beads compared to centrifugation & removal of buffers in a V-well plate. |
| Question | Answer |
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How do I analyse my data? |
We can analyse your data for free if your FCS files are compatible with FCAP Array V3. |
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Can I use FlowJo to carry out my data analysis? |
Yes. You can use FlowJo to obtain PE MFIs (median fluorescence intensity) of each bead set then use a software package (e.g. Prism or https://elisaanalysis.com/) that can perform nonlinear regression analysis to carry out curve fitting and concentration calculations. |
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How does GeniePlex provide a fully quantitative signal? |
Similar to the principle of a sandwich ELISA, each bead population, conjugated with a specific capture antibody, traps the protein of interest, such as a cytokine, in the sample. The amount of the analyte captured is detected via a biotinylated antibody against a secondary epitope of the protein, followed by a streptavidin-R-phycoerythrin treatment. The fluorescent intensity of R-phycoerythrin on the beads is quantified on a flow cytometer equipped with a 488nm laser and measured using the PE channel detector. |
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Is there still some kind of standard curve used? And if yes, is this one standard curve including all the possible analytes that I want to analyse in several dilutions? |
Concentrations of the protein of interest in the samples can be obtained by comparing the fluorescent signals to those of a standard curve generated from a serial dilution of a known concentration of the protein analyte. These are multiplex assays. Analytes of interest are measured simultaneously in a single reaction. |
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My flow cytometer is Facs Aria II and I use Flow Jo software. Would you recommend that I use an alternative software? |
We recommend FCAP Array V3 from BD or FCAP Array Infinite from SoftFlow. But, as indicated above, Flow Jo can be used if preferred. |
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Are LMD files from (Beckman Coulter) compatible with FCAP Array? |
There are 3 cytometer models from Beckman Coulter, FC500, Navios and Galios. Navios and Galios are essentially the same. Navios is CE marked and Galios is for research use only. We tested lmd files from Navios and Galios in the past without any issues as long as scales settings of PE and APC channels are changed from default 10^-1 (i.e. 0.1) to 10^0 (i.e. 1.0) (under "File" > "Edit FCS Attributes"). |
| Question | Answer |
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Should the unused wells be directly sealed with the plate seal? |
Yes. Seal the unused wells with a plate seal. It is important to place the filter plate on top of the filter plate lid (inverted) during the entire assay process to prevent touching the plate bottom on any surface other than tapping the plate bottom onto several layers of paper towels to remove residual buffers in Steps 5, 14, 21 and 28. The purpose is to avoid potential contamination. |
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Can GeniePlex be integrated with a liquid handling system? |
Yes. Please reach out to our team for further details. |
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Can the GeniePlex Protocol be automated? |
GeniePlex assays are validated only for manual processes. If you would like to automate GeniePlex to your process, you would need to optimise on your own system. We are happy to help with this. |
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Can you use an ELISA plate reader? |
No. ELISA plate reader does not work for GeniePlex assays. You need to use a flow cytometer to read GeniePlex assays. |
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Are GeniePlex assays amenable to High-throughput systems? |
In order for GeniePlex assays to be amenable to high-throughput and automated liquid handling systems, we develop special assays that use magnetic beads in a 384-well format. Our 384-well assays are not catalog products as they are primarily custom projects. Similar to flow cytometers, there are many models of liquid handling systems from a number of automation companies (Beckman, Tecan, Hamilton to name a few). A protocol working on one model may, or certainly, not working on another. As a result, each customer will need to validate our assay on their specific system. Our Head of Assay Development can assist and answer any questions here. The flow cytometry analysis part is very much the same as the current non-magnetic beads protocol (see attached). The bead size is about the same, 4-5 microns. Beads can be identified/classified on the APC (excited with a red laser) channel. Assay reporter is the same as the current one, PE. Since the beads are magnetic, a regular (not filter) 96-well or 384-well plate in conjunction with a magnetic plate washer can be used to run an assay. It is easier to be automated by using, for example, an automated plate washer. |
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I thought that flow cytometers mainly separated cells by the scattering of light. How do they separate the beads based on their size and does it require a specific setting in the flow cytometer? |
The GeniePlex multiplex assay technology utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With two sizes of beads of 4 and 5 microns and twelve levels of fluorescence intensity in each bead size (4S and 5S), the AimPlex technology can measure up to 24 analytes simultaneously in a single reaction using only 15µL of sample. The bead populations in the reaction are determined using a standard flow cytometer equipped with a blue 488nm and red 635nm laser or with only a 488nm laser. The maximum emission of the bead classification dye is at 700 nm. |
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What is the recommended flow rate for running the samples, should they run on low or medium? |
Please run on medium |
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What if I am working with both serum and CCS samples? |
If you want to run serum samples and cell culture supernatant samples at the same time, the SPB (Serum, Plasma, Bodily Fluid) Diluent Kit can be used. The SPB Diluent kit can be used for cell culture supernatant (CCS) samples. There are extra components in the SPB Diluent Kit to minimize matrix effects in serum, plasma and bodily fluid samples. Those components have no effect on CCS samples because CCS are less complex matrices. |
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Would you recommend running samples in duplicate or triplicate? |
Similar to ELISA, duplicates are recommended. |
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How does the Flow cytometer detect analytes? |
Similar to the principle of a sandwich ELISA, each bead population, conjugated with a specific capture antibody, traps the protein of interest, such as a cytokine, in the sample. The amount of the analyte captured is detected via a biotinylated antibody against a secondary epitope of the protein, followed by a streptavidin-R-phycoerythrin treatment. The fluorescent intensity of R-phycoerythrin on the beads is quantified on a flow cytometer equipped with a 488nm laser and measured using the PE channel detector. |
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What are the advantages associated with the wash steps? |
The wash step after cAb-Ag binding and before adding biotinylated dAb avoids a false-negative hook effect in the presence of a large excess of measured analytes. We have found that with wash steps, in many cases, assay backgrounds are lower and assay sensitivities are higher. |
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What are the steps involved in running a partial plate? |
Page 16: " 7.2 Premixed Multiplex Kits or Kits Packaged in a Premixed Format IMPORTANT: Prepare a working solution by transferring the entire content of the 2x NR or Mouse/Rat dAb diluent to the 2x Biotin-dAb vial. Mix by gentle vortexing. The working solution can be stored at 2-8oC for up to 24 hrs. NOTE: If running a partial plate, calculate the amount of the working solution needed by multiplying the number of wells by 25 µL. We recommend adding an additional 2 wells to account for pipetting recovery. For example, if a total of 48 wells are needed in the experiment, prepare enough for 50 wells (50 wells × 25 µL = 1,250 µL total working detection antibody solution). Prepare the working detection antibody solution by combining 625 µL of 2x NR or Mouse/Rat dAb diluent and 625 µL of the 2x Premixed Biotin-dAbs. " Page 17: "8 Preparing Antigen Standards Note: Reconstitute and prepare serial dilutions of the antigen standards no more than 2 hours before carrying out the assay(s) in Section 9. Discard after use. Freezing, thawing and reuse of the re-constituted and diluted standards are NOT recommended. If running a partial plate, extra vials of antigen standards should be ordered." Page 18: " 9.1. Prepare the filter plate template. Mark the standard, sample and blank wells. Standards and samples should be run in duplicates or triplicates. If the whole plate will not be used, seal the unused well with a plate seal." Also, if you wish to run a partial GeniePlex plate, there is only one vial of lyophilized standard mix in each kit. If multiple runs are needed, corresponding numbers of extra vial(s) of standard mix need to be ordered. |
| Question | Answer |
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Would you recommend FACS Aria over BC FC500 machine? |
It acquires samples faster and no need to adjust scale settings for FCAP Array v3 analysis I mentioned earlier. Furthermore, blue and red lasers on the Aria are spatially separated. There is no need for any color compensation between PE and APC. On the other hand, blue and red lasers on the FC500 are collinear. Some color compensation, although a very small percentage, is usually needed between PE and APC. |
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How do I analyse my data using a BC FC500? |
If you would like us to use FCAP Array V3 to analyze this data, it is very important to change log scale settings of PE and APC channels on the FC500 from default 10^-1 (i.e. 0.1) to 10^0 (i.e. 1.0) (under "File" > "Edit FCS Attributes"). The reason for this change is because FCAP Array V3 cannot handle fluorescent values <1.0 well. Please make sure both Areas (Integral) and Heights (Peak) of the FL parameters are collected. |
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How long can my assayed plate be stored before running on the cytometer? |
We have found that an assayed plate can be stored for 16 hours at 2-8C in the dark (please refer to the NOTE of Section 9.33 in the User Manual. An assayed plate should be stable for ~3 hours at room temperature if protected from light. |
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Can GeniePlex assays be run on Guava cytometers? |
We do not recommend Guava cytometers for GeniePlex assays. Guava instruments can compromise/interfere with the high sensitivity of GeniePlex assays. |
| Question | Answer |
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What QC process is involved in GeniePlex assays? |
Bead Population QC Bead Clustering: 1 bead cluster on FS/SS dot plot = PASS Peak Test: E.g. One bead peak on the "APC" or "PE-Cy5" channel of 5 micron beads.
Assay QC Standard Dose Recovery Testing: 70-130% = PASS. Sensitivity Testing (LOD): Assay dependant, e.g. <5 pg/ml. Standard Curve: MFI/Recovery % testing. Positive Controls: We do not provide assay controls since the majority of our orders are custom panels. It is way too expensive to provide controls for custom panels. For a longer term study that will last a year to 2, there are a few possible approaches that we are happy to support. 1. We can reserve the same lots of key reagents (standard mix, cAb-beads, biotin-dAbs and SAPE) for the entirety projects upon request. Therefore, lot-to-lot variations are minimized. The standards can be served as controls. 2. You can make high, mid and low-level controls for the assay panel. 3. We can also make high, mid and low-level controls for the assay panel for you upon request. There will be a cost associated with this depending on the number of analytes and quantities. Cross-Reactivity Testing: All pre-made and custom GeniePlex assays are tested for cross-reactivity |
Citations
| Author(s) | Title | Journal | Kit |
| Al-Hourani et al. | Innate triggering and antiviral effector functions of activin A | Wellcome Open Research | Custom GeniePlex |
| Chen et al. | HBO-PC Promotes Locomotor Recovery by Reducing Apoptosis and Inflammation in SCI Rats: The Role of the mTOR Signaling Pathway | Cellular and Molecular Neurobiology | GeniePlex Rat IL-1b Immunoassay |
| Van Os et al. | Modelling fatty liver disease with mouse liver-derived multicellular spheroids | Biomaterials | GeniePlex Mouse Inflammation 5-Plex Panel 1 (96 Tests) |
GeniePlex Success Stories
Support Based Multiplexing!
GeniePlex is a Multiplex technology that guarantees reliable and reproducible data.
How do we guarantee this?
Support at every stage throughout your project.
Case Study: Aobhín Sheedy, PhD Candidate Biomedical Engineering, University of Galway
Custom Human GeniePlex 6-Plex Assay
TNFSF10/sCD253/TRAIL, IFN-gamma, Granzyme B/GZYMB/CTL1, TNFSF6/sCD178/sCD95L/sFasL, Granzyme A/GZYMA/CTL3A, and TNF-alpha
A schematic of our GeniePlex Customer Support workflow.
1. Design & Development: Our technical team and the researchers in the University of Galway spent a number of weeks designing and developing a custom panel that best fitted Aobhín's research requirements.
2. Order & Delivery: The research project was time-sensitive and the custom panel was delivered in 7 business days.
3. Training Session 1 - Flow Cytometer Set-Up: It was Aobhín's first time to run GeniePlex, so we scheduled a 1 hour training session with her team, our Assay Specialist, Shane, and our technical director, Yong. An acquisition protocol was set up and questions were answered in real time.
4. Training Session 2 - Sample Run: In order to ensure the sample run went smoothly, we joined a quick zoom to answer a few questions regarding gating, naming of samples, and standard curve.
5. Training Session 3 - Data Analysis: As part of GeniePlex assays, we offer complementary data analysis, so we did this for Aobhín. After a short back and forth correcting some settings from the sample run, we completed the data analysis report and sent it to Aobhín (see attached).