Adrenocorticotropic hormone (ACTH) is a peptide hormone that is secreted by the anterior pituitary gland and stimulates the production and release of glucocorticoids from the adrenal cortex. The ACTH is released in response to a stressor, such as an exercise, hypoglycemia, or pain. ACTH plays a key role in the function of the sympathetic nervous system. It is used as a marker for stress and has been associated with the symptoms of chronic stress such as anxiety, depression, and insomnia. The most common cause of ACTH deficiency is surgical removal or destruction of part or all of one or both adrenal glands.
Description
Human ACTH ELISA Kit
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Human ACTH ELISA Kit |
| Product Code: | HUFI00819 |
| Size: | 96 Assays |
| Alias: | ACTH, Adrenocorticotropic Hormone, Corticotropin, Adrenocorticotropin |
| Detection Method: | Competitive ELISA, Coated with Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human ACTH concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 9.375pg/ml |
| Range: | 15.625-1000pg/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery: | Matrices listed below were spiked with certain level of Human ACTH and the recovery rates were calculated by comparing the measured value to the expected amount of Human ACTH in samples.
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ACTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer |
