The Human ADM (Adrenomedullin) ELISA Kit is specifically designed for the precise measurement of adrenomedullin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it a valuable tool for various research purposes.Adrenomedullin is a key regulatory peptide that plays a vital role in numerous physiological processes, including vasodilation, cardiovascular homeostasis, and anti-inflammatory effects. Dysregulation of adrenomedullin has been associated with various diseases such as sepsis, heart failure, and pulmonary hypertension, highlighting its significance as a biomarker for disease research and therapeutic development.
The Human ADM ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for measuring adrenomedullin levels in human samples, facilitating in-depth studies on the role of adrenomedullin in health and disease. With its reliable performance and user-friendly protocol, this kit is an indispensable tool for advancing scientific research in the field of adrenomedullin biology.
Product Name:
Human ADM (Adrenomedullin) ELISA Kit
SKU:
HUES01511
Target:
Human ADM (Adrenomedullin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADM. You can calculate the concentration of Human ADM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
90-100
95-109
Average (%)
92
95
102
1:4
Range (%)
93-110
84-96
88-99
Average (%)
100
91
94
1:8
Range (%)
88-102
86-100
84-96
Average (%)
93
93
91
1:16
Range (%)
90-103
85-96
87-98
Average (%)
97
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
102
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.86
87.6
370.81
50.94
79.2
389.34
Standard deviation
3.17
4.96
12.68
3.04
4.28
14.06
C V (%)
6.49
5.66
3.42
5.97
5.4
3.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ADM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADM in samples. No significant cross-reactivity or interference between Human ADM and analogues was observed.
