The Human Beta-EP (Beta-Endorphin) ELISA Kit is a reliable and sensitive tool for accurately measuring beta-endorphin levels in human biological samples. This kit is designed for use with serum, plasma, and cell culture supernatants, providing researchers with consistent and reproducible results.Beta-endorphin is a key peptide hormone known for its role in regulating pain perception, stress response, and mood. Dysregulation of beta-endorphin levels has been linked to various disorders such as chronic pain, mood disorders, and addictive behaviors. By accurately measuring beta-endorphin levels, researchers can gain valuable insights into these conditions and potentially develop targeted therapies.With its high sensitivity and specificity, the Human Beta-EP ELISA Kit is an essential tool for studying the physiological and pathological roles of beta-endorphin in human health and disease. Explore the potential of beta-endorphin research with this advanced ELISA kit from Assay Genie.
Product Name:
Human beta-EP (Beta-Endorphin) ELISA Kit
SKU:
HUES01751
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
