Description
Human Cancer Discovery Array (30 targets) (SARB0029)
The Human Cancer Discovery Array (SARB0029) from Assay Genie is a comprehensive research tool designed to investigate 30 key targets involved in cancer progression and tumor development. This array includes antibodies against a variety of proteins implicated in cancer pathways, allowing for the simultaneous analysis of multiple targets in a single experiment.With validated antibodies for targets such as growth factors, transcription factors, and cell cycle regulators, researchers can gain valuable insights into the molecular mechanisms driving cancer growth and metastasis. The array is suitable for use in various experimental techniques, including Western blotting and immunohistochemistry, making it a versatile tool for cancer research studies.
By enabling the simultaneous detection of multiple cancer-related targets, the Human Cancer Discovery Array streamlines the research process and helps researchers identify potential biomarkers or therapeutic targets for further investigation. Whether studying tumor heterogeneity, drug resistance mechanisms, or tumor microenvironment interactions, this array provides a valuable resource for advancing our understanding of cancer biology and developing new strategies for cancer diagnosis and treatment.
| Product Sku: | SARB0029 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 30 |
| Gene Symbols: | AFP, B2M, CA (19-9), CCL5, CEACAM5, CGA, CRP, CXCL8, DKK1, ENO2|ERBB2, FGF2, FSHB, FTH1, GDF15, HGF, ICAM1, KITLG, KLK3, LGALS3, MIF, MMP2, MUC16, PRAP1, SERPINE1, SPP1, TGFB1, THBS1, TIMP1, TNFRSF11B, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Alpha-fetoprotein
| Beta-2 Microglobulin
| bFGF
| CA125
| CA19-9
|
CEA
| CRP (C-Reactive Protein)
| DKK-1
| ErbB2
| Ferritin
|
FSH
| Galectin-3
| GDF-15
| HGF
| ICAM-1 (CD54)
|
IL-8 (CXCL8)
| MIF
| MMP-2
| NSE
| Osteoprotegerin (TNFRSF11B)
|
Osteopontin (SPP1)
| PAI-1
| PSA-free
| RANTES (CCL5)
| SCF
|
TGF beta 1
| Thrombospondin 1
| TIMP-1
| uPA
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cancer Discovery Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
