Human COL1 alpha2 (Collagen Type I Alpha 2) ELISA Kit
The Human COL1A2 (Collagen Type I Alpha 2) ELISA Kit is specifically designed for the precise measurement of collagen type I alpha 2 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and dependable results for a variety of research applications.Collagen type I alpha 2 is a vital component of the extracellular matrix, contributing to the structural integrity of tissues such as skin, tendons, and bone. Dysregulation of collagen synthesis and degradation is associated with numerous pathological conditions, including fibrosis, arthritis, and skin disorders. Therefore, this ELISA kit serves as a valuable tool for investigating collagen-related diseases and developing potential therapeutic interventions.
Product Name:
Human COL1 alpha2 (Collagen Type I Alpha 2) ELISA Kit
SKU:
HUES01914
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
