Description
system_update_altDatasheet
Human Cytokine Array (174 targets) (SARB0036)
The Human Cytokine Array (Sar038) is a powerful tool for researchers studying the functions of various cytokines in biological samples. With the ability to detect 174 different cytokine targets simultaneously, this array offers a comprehensive and efficient way to analyze cytokine expression profiles. This array is suitable for a wide range of applications, including immunology, inflammation, cancer research, and drug discovery. By providing a snapshot of the cytokine profile in a sample, researchers can gain valuable insights into the immune response, disease progression, and potential therapeutic targets.
With high sensitivity and specificity, the Human Cytokine Array (Sar038) offers reliable and reproducible results, making it an essential tool for advancing research in the field of cytokine biology. Explore the diverse range of cytokine targets available in this array and unlock new discoveries in your research.
| Product Sku: | SARB0036 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 174 |
| Gene Symbols: | ADIPOQ, AGRP, ALCAM, ANG, ANGPT2, AREG, AXL, BDNF, BMP4, BMP5|BMP6, BMP7, BTC, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD14, CD80, CDH5, CNTF, CSF1, CSF1R, CSF2, CSF3, CTF1, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, EGF, EGFR, ENG, ERBB3, FAS, FASLG, FGF2, FGF4, FGF6, FGF7, FGF9, FIGF, FLT3LG, FLT4, GDNF, HGF, ICAM1, ICAM2, ICAM3, IFNG, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP6, IL10, IL10RB, IL11, IL12A, IL12B, IL13, IL13RA2, IL15, IL16, IL17A, IL18BP, IL18RAP, IL1A, IL1B, IL1R1, IL1R2, IL1RL1, IL1RN, IL2, IL21R, IL2RA, IL2RB, IL2RG, IL3, IL4, IL5, IL5RA, IL6, IL6R, IL6ST, IL7, IL9, INHBA, KDR, KIT, KITLG, LEP, LEPR, LIF, LTA, MIF, MMP1, MMP13, MMP3, MMP9, MST1, NGF, NGFR, NTF3, NTF4, OSM, PDGFA, PDGFB, PDGFRA, PDGFRB, PECAM1, PGF, PLAUR, PPBP, PRL, SELE, SELL, SIGLEC5, TEK, TGFA, TGFB1, TGFB2, TGFB3, THPO, TIE1, TIMP1, TIMP2, TIMP4, TNF, TNFRSF10C, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF1A, TNFRSF1B, TNFRSF21, TNFSF14, TNFSF18, TPO, TYRO3, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Adiponectin (ACRP30)
| Activin A
| AgRP
| ALCAM (CD166)
| Amphiregulin
|
Angiogenin
| Angiopoietin-2
| Axl
| CD80 (B7-1)
| BDNF
|
bFGF
| BLC (CXCL13)
| BMP-4
| BMP-5
| BMP-6
|
BMP-7
| beta-NGF
| Betacellulin (BTC)
| Cardiotrophin-1 (CT-1)
| CCL28 (MEC)
|
CD14
| Ck beta 8-1 (CCL23)
| CNTF
| CTACK (CCL27)
| CXCL16
|
DR6 (TNFRSF21)
| Dtk
| EGF
| EGFR
| ENA-78 (CXCL5)
|
Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| ErbB3
|
E-Selectin
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
| FGF-4
| FGF-6
|
FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| GCP-2 (CXCL6)
|
GCSF
| GDNF
| GITR (TNFRSF18)
| GITR Ligand (TNFSF18)
| GM-CSF
|
GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| HCC-4 (CCL16)
| HGF
| I-309 (TCA-3/CCL1)
|
ICAM-1 (CD54)
| ICAM-2 (CD102)
| ICAM-3 (CD50)
| IFN-gamma
| IGFBP-1
|
IGFBP-2
| IGFBP-3
| IGFBP-4
| IGFBP-6
| IGF-1
|
IGF-1 R
| IGF-2
| IL-1 R2
| IL-1 R4 (ST2)
| IL-1 R1
|
IL-10
| IL-10 R beta
| IL-11
| IL-12 p40
| IL-12 p70
|
IL-13
| IL-13 R alpha 2
| IL-15
| IL-16
| IL-17A
|
IL-18 BP alpha
| IL-18 R beta (AcPL)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
|
IL-2
| IL-2 R beta (CD122)
| IL-2 R gamma (Common gamma Chain)
| IL-2 R alpha
| IL-21 R
|
IL-3
| IL-4
| IL-5
| IL-5 R alpha
| IL-6
|
IL-6 R
| IL-7
| IL-8 (CXCL8)
| IL-9
| IP-10 (CXCL10)
|
I-TAC (CXCL11)
| LAP/TGF beta 1
| Leptin
| Leptin R
| LIF
|
Light (TNFSF14)
| L-Selectin (CD62L)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
|
MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| M-CSF R
| MDC (CCL22)
|
MIF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
|
MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
| MMP-1
| MMP-13
| MMP-3
|
MMP-9
| MPIF-1 (CCL23)
| MSP alpha/beta
| NAP-2 (PPBP/CXCL7)
| NGFR (TNFRSF16)
|
NT-3
| NT-4
| Oncostatin M
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
|
PDGF-AA
| PDGF R alpha
| PDGF R beta
| PDGF-AB
| PDGF-BB
|
PECAM-1 (CD31)
| PLGF
| Prolactin
| RANTES (CCL5)
| SCF
|
SCF R (CD117/c-kit)
| SDF-1 alpha (CXCL12 alpha)
| SDF-1 beta (CXCL12 beta)
| gp130
| Siglec-5 (CD170)
|
TNF RII (TNFRSF1B)
| TNF RI (TNFRSF1A)
| TARC (CCL17)
| TECK (CCL25)
| TGF beta 2
|
TGF alpha
| TGF beta 3
| TGF beta 1
| Thrombopoietin (TPO)
| Tie-1
|
Tie-2
| TIMP-1
| TIMP-2
| TIMP-4
| TNF alpha
|
TNF beta (TNFSF1B)
| TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| uPAR
| VE-Cadherin (CDH5)
|
VEGF-A
| VEGFR2
| VEGFR3
| VEGF-D
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C2000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
