Human IGF-1 (Insulin-Like Growth Factor 1) CLIA Kit
The Human IGF-1 (Insulin-like Growth Factor 1) CLIA Kit is a cutting-edge tool for the accurate measurement of IGF-1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results that are vital for various research applications.IGF-1 is a key growth factor that plays a crucial role in cell growth, differentiation, and metabolism. It is involved in numerous physiological processes, including muscle growth, tissue repair, and bone density regulation.
Dysregulation of IGF-1 levels has been linked to various diseases such as diabetes, cancer, and growth disorders, highlighting its importance as a valuable biomarker for diagnostic and therapeutic purposes.With its advanced technology and reliable performance, the Human IGF-1 CLIA Kit is an indispensable tool for researchers and healthcare professionals studying IGF-1 biology and its implications in human health and disease.
Product Name:
Human IGF-1 (Insulin-Like Growth Factor 1) CLIA Kit
SKU:
HUES00083
Target:
Human IGF-1 (Insulin-Like Growth Factor 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGF-1. You can calculate the concentration of Human IGF-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
97-111
92-104
Average (%)
103
105
98
1:4
Range (%)
99-113
88-101
95-109
Average (%)
106
94
101
1:8
Range (%)
87-101
98-113
97-110
Average (%)
94
106
103
1:16
Range (%)
96-111
100-114
89-104
Average (%)
104
108
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
99-113
107
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.47
7.82
0.96
2.27
7.54
Standard deviation
0.1
0.18
0.75
0.1
0.19
0.71
C V (%)
10.31
7.29
9.59
10.42
8.37
9.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGF-1 in samples. No significant cross-reactivity or interference between Human IGF-1 and analogues was observed.
