Description
Human/Mouse MAPK Phosphorylation Array (17 targets) (SARB0055)
The Human/Mouse MAPK Phosphorylation Array (SARB0055) is a powerful tool for studying signal transduction pathways involved in cellular responses to various stimuli. This array allows for the simultaneous detection and quantification of phosphorylation levels of 17 key proteins involved in the MAPK pathway, including MAPKs, MAPK kinases, and MAPK substrates.With high sensitivity and specificity, this array can provide valuable insights into the activation status of MAPK signaling cascades in response to different experimental conditions.
It is suitable for use with both human and mouse samples, making it a versatile tool for researchers studying MAPK signaling in a variety of biological systems.This array is a reliable and cost-effective option for profiling the phosphorylation status of multiple MAPK pathway proteins in a single experiment. It is an essential tool for researchers studying cell signaling pathways, drug discovery, and biomarker identification in the fields of cancer, inflammation, and neurobiology.
| Product Sku: | SARB0055 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human / Mouse |
| Number of Targets Detected: | 17 |
| Gene Symbols: | AKT1, CREB1, GSK3A, GSK3B, HSPB1, MAP2K1, MAP2K3, MAP2K6, MAPK1, MAPK14|MAPK8, MTOR, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KB1, TP53 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / HER/ErbB Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / NFkB Signaling / PI3K-AKT Signaling / Wnt/beta-Catenin Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Akt (P-Ser473) (PKB (P-Ser473))
| CREB (P-Ser133)
| ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
| GSK3a (P-Ser21)
| GSK3b (P-Ser9)
|
HSP27 (P-Ser82)
| JNK (P-Thr183)
| MEK (P-Ser217/Ser221)
| MKK3 (P-Ser189)
| MKK6 (P-Ser207)
|
MSK2 (P-Ser360)
| mTOR (P-Ser2448)
| p38 (P-Thr180/Tyr182)
| P53 (P-Ser15)
| P70S6K (P-Thr421/Ser424)
|
RSK1 (P-Ser380)
| RSK2 (P-Ser386)
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit-IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
