Human SDF-1 (Stromal Cell Derived Factor 1) CLIA Kit
The Human SDF-1 (Stromal Cell-Derived Factor 1) CLIA Kit is a highly efficient assay kit designed for the precise measurement of SDF-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.SDF-1, also known as CXCL12, is a critical chemokine involved in various biological processes, including stem cell homing, immune response modulation, and tissue repair. Dysregulation of SDF-1 has been linked to several diseases, including cancer, cardiovascular disorders, and inflammatory conditions, highlighting its importance as a potential diagnostic and therapeutic target.
By utilizing the Human SDF-1 CLIA Kit, researchers can gain valuable insights into the role of SDF-1 in physiological and pathological processes, paving the way for the development of novel treatments and interventions. Trust in the accuracy and reliability of this kit to support your studies and advance our understanding of SDF-1 biology.
Product Name:
Human SDF-1 (Stromal Cell Derived Factor 1) CLIA Kit
SKU:
HUES00050
Target:
Human SDF-1 (Stromal Cell Derived Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SDF-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SDF-1. You can calculate the concentration of Human SDF-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
96-114
91-106
Average (%)
102
104
97
1:4
Range (%)
98-115
104-117
88-101
Average (%)
106
110
95
1:8
Range (%)
88-103
84-97
102-115
Average (%)
96
90
109
1:16
Range (%)
101-116
102-119
97-111
Average (%)
107
110
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-116
108
EDTA plasma (n=5)
99-113
107
Cell culture media (n=5)
94-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.61
271.56
923.29
95.05
285.78
961.32
Standard deviation
8.51
23.3
61.68
11.08
24.23
95.75
C V (%)
8.9
8.58
6.68
11.66
8.48
9.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SDF-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SDF-1 in samples. No significant cross-reactivity or interference between Human SDF-1 and analogues was observed.
