The [KO Validated] SOX2 Monoclonal Antibody (CAB19118) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been rigorously validated for knockout (KO) studies and is suitable for use in techniques such as immunofluorescence and immunohistochemistry.SOX2, a member of the SRY-related HMG-box (SOX) family of transcription factors, plays a crucial role in maintaining the self-renewal and differentiation of stem cells. It is also implicated in various cancers, where its expression levels can impact tumor growth and progression.
This antibody is validated for use in WB, ELISA, IF-F applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
[KO Validated] SOX2 Monoclonal Antibody
SKU:
CAB19118
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0449
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MYNM METE LKPP GPQQ TSGG GGGN STAA AAGG NQKN SPDR VKRP MNAF MVWS RGQR RKMA QENP KMHN SEIS KRLG AEWK LLSE TEKR PFID EAKR LRAL
Tested Applications:
WBELISAIF-F
Recommended Dilution:
WB
1:1000 - 1:2000
IF-F
1:100 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ANOP3, MCOPS3, SOX2
Positive Sample:
Mouse brain, Rat brain, 293T
Cellular Localization:
Nucleus.
Calculated MW:
34kDa
Observed MW:
35kDa
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Purification Method
Affinity purification
Gene ID
6657
RRID
AB_2862611
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SOX2 Rabbit mAb (CAB19118) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal imaging of mouse brain(frozen section) using SOX2 Rabbit mAb (CAB19118,at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
