The Monkey C5a (Complement Component 5a) ELISA Kit is a powerful tool for accurate measurement of C5a levels in monkey serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it perfectly suited for a variety of research applications.C5a is a key component of the complement system, playing a crucial role in inflammation, immune response, and host defense. Dysregulation of C5a has been implicated in various diseases, including sepsis, autoimmune disorders, and inflammatory conditions. By accurately measuring C5a levels, researchers can gain valuable insights into the mechanisms underlying these pathological processes and explore new therapeutic targets.With the Monkey C5a ELISA Kit, researchers can confidently study the role of C5a in monkey models, furthering our understanding of immune regulation and inflammatory diseases. Don't miss out on this essential tool for your research endeavors.
Product Name:
Monkey C5a (Complement Component 5a) ELISA Kit
SKU:
MKES00048
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
