The Mouse Beta-EP (Beta-Endorphin) ELISA Kit is specifically designed for the accurate quantification of beta-endorphin levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. Beta-endorphin is a key neuropeptide involved in pain modulation, stress response, and mood regulation.
It plays a critical role in various physiological processes and has been implicated in conditions such as chronic pain, addiction, and mood disorders. By measuring beta-endorphin levels, researchers can gain valuable insights into these complex mechanisms and potential therapeutic interventions.
Product Name:
Mouse beta-EP (Beta-Endorphin) ELISA Kit
SKU:
MOES00767
Target:
Mouse beta-EP (Beta-Endorphin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-EP. During the reaction, β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
93-103
86-97
Average (%)
98
98
91
1:4
Range (%)
83-97
86-102
100-114
Average (%)
90
93
106
1:8
Range (%)
86-100
86-101
94-106
Average (%)
93
93
99
1:16
Range (%)
85-96
93-103
96-108
Average (%)
90
98
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.4
99.8
404.5
51.1
94.9
411.3
Standard deviation
2.9
5.5
14.2
3.2
5.4
14.8
C V (%)
5.87
5.51
3.51
6.26
5.69
3.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of β-EP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.
