Description
system_update_altDatasheet
Mouse Cytokine Array (97 targets) (SARB0084)
The Mouse Cytokine Array (97 targets) is a high-throughput screening tool designed for researchers investigating cytokine expression levels in biological samples. With 97 unique targets, this array allows for the simultaneous detection of a wide range of cytokines, providing a comprehensive overview of the immune response and inflammatory processes.This array is compatible with mouse samples and can be utilized in a variety of applications, including serum, plasma, cell culture supernatants, and tissue lysates. The sensitivity and specificity of the array ensure accurate and reliable results, making it an invaluable tool for studies in immunology, infectious diseases, and autoimmune disorders.
Researchers can use the Mouse Cytokine Array to identify cytokine profiles associated with different disease states, monitor changes in cytokine expression over time, and unravel complex signaling pathways involved in immune regulation. By analyzing multiple cytokines in a single experiment, researchers can save time and resources while gaining valuable insights into the immune system's response to various stimuli.Overall, the Mouse Cytokine Array (97 targets) is a versatile and powerful tool for researchers seeking to explore the intricate network of cytokine signaling and its impact on health and disease.
| Product Sku: | SARB0084 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 97 |
| Gene Symbols: | AREG, AXL, CCL11, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL24|CCL3, CCL5, CCL9, CD27, CD36, CD70, CDH1, CSF3, CTF1, CTLA4, CX3CL1, CXCL1, CXCL11, CXCL13, CXCL15, CXCL16, CXCL2, CXCL9, DCN, DKK1, EGF, EPGN, F11R, FASLG, FCGR2B, FIGF, FLT1, FLT3LG, FLT4, GAS6, GZMB, HGF, IFNG, IGF2, IGFBP5, IGFBP6, IL10, IL11, IL12A, IL12B, IL13, IL15, IL17A, IL17F, IL17RB, IL1A, IL1B, IL1RN, IL2, IL20, IL21, IL25, IL2RA, IL3, IL4, IL5, IL6, IL9, KITLG, LEP, LEPR, LGALS1, MADCAM1, MFGE8, MMP2, MMP9, PRL, SELE, SELL, SLPI, SPINT1, SPP1, THPO, TNF, TNFRSF11B, TNFRSF13B, TNFRSF18, TNFRSF19, TNFRSF8, TNFSF11, TNFSF12, TNFSF18, TNFSF8, TPO, VCAM1, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
6Ckine (CCL21)
| ALK-1
| Amphiregulin
| Axl
| BLC (CXCL13)
|
Cardiotrophin-1 (CT-1)
| CD27 (TNFRSF7)
| CD27 Ligand (TNFSF7)
| CD30 (TNFRSF8)
| CD30 Ligand (TNFSF8)
|
CD36 (SR-B3)
| CTLA-4 (CD152)
| CXCL16
| Decorin
| DKK-1
|
E-Cadherin
| EGF
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Epigen
|
E-Selectin
| Fas Ligand (TNFSF6)
| Fc gamma RIIB (CD32b)
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
Galectin-1
| Gas 6
| GCSF
| GITR (TNFRSF18)
| GITR Ligand (TNFSF18)
|
Granzyme B
| HAI-1
| HGF
| IFN-gamma
| IGFBP-5
|
IGFBP-6
| IGF-2
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
|
IL-2
| IL-2 R alpha
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-9
| IL-10
| IL-11
| IL-12 p40
|
IL-12 p70
| IL-13
| IL-15
| IL-17A
| IL-17 RB
|
IL-17E (IL-25)
| IL-17F
| IL-20
| IL-21
| I-TAC (CXCL11)
|
JAM-A (CD321/F11R)
| KC (CXCL1)
| Leptin
| Leptin R
| L-Selectin (CD62L)
|
Lungkine (CXCL15)
| MAdCAM-1
| MCP-1 (CCL2)
| MDC (CCL22)
| MFG-E8
|
MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 gamma
| MIP-2
| MIP-3 alpha (CCL20)
|
MIP-3 beta (CCL19)
| MMP-2
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| Prolactin
|
Pro-MMP-9
| RANTES (CCL5)
| SCF
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
|
TACI (TNFRSF13B)
| TARC (CCL17)
| TNF alpha
| Thrombopoietin (TPO)
| TRANCE (TNFSF11)
|
TROY (TNFRSF19)
| TWEAK R (TNFRSF12)
| VCAM-1 (CD106)
| VEGF-A
| VEGFR1
|
VEGFR3
| VEGF-D
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C6 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 4-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
