Mouse HMGB-1 (High mobility group protein B1) ELISA Kit
The Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit is specifically designed for the accurate and reliable detection of HMGB-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.HMGB-1 is a key protein involved in inflammation, immune response, and tissue repair. It plays a critical role in various diseases, including sepsis, arthritis, and cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its advanced technology and optimized protocols, the Mouse HMGB-1 ELISA Kit from Assay Genie provides researchers with a powerful tool to study HMGB-1 biology and its implications in disease pathogenesis. Order now and accelerate your research with confidence.
Product Name:
Mouse HMGB-1 (High mobility group protein B1) ELISA Kit
SKU:
MOES01177
Target:
Mouse HMGB-1 (High mobility group protein B1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HMGB-1. You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
96-110
87-97
Average (%)
97
103
92
1:4
Range (%)
90-104
84-98
85-98
Average (%)
97
90
91
1:8
Range (%)
90-101
84-96
82-95
Average (%)
95
89
88
1:16
Range (%)
90-106
81-95
82-94
Average (%)
98
87
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
83-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.5
98.56
334.18
52.21
95.09
337.87
Standard deviation
2.65
4.51
17.78
3.53
5.3
13.18
C V (%)
5.25
4.58
5.32
6.76
5.57
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse HMGB-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HMGB-1 in samples. No significant cross-reactivity or interference between Mouse HMGB-1 and analogues was observed.
