Mouse HMGB-1 (High mobility group protein B1) ELISA Kit
The Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit is specifically designed for the accurate and reliable detection of HMGB-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.HMGB-1 is a key protein involved in inflammation, immune response, and tissue repair. It plays a critical role in various diseases, including sepsis, arthritis, and cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. With its advanced technology and optimized protocols, the Mouse HMGB-1 ELISA Kit from Assay Genie provides researchers with a powerful tool to study HMGB-1 biology and its implications in disease pathogenesis. Order now and accelerate your research with confidence.
Product Name:
Mouse HMGB-1 (High mobility group protein B1) ELISA Kit
SKU:
MOES01177
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
