The Mouse HSP 70 (Heat Shock Protein 70) ELISA Kit is specifically designed for precise and accurate detection of HSP 70 levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing dependable and consistent results for various research applications.HSP 70 is a key player in cellular stress response, helping to maintain protein homeostasis and protect cells from damage. Its role in various physiological and pathological processes, including cancer, inflammation, and neurodegenerative diseases, makes it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.
With easy-to-follow protocols and user-friendly features, the Mouse HSP 70 ELISA Kit from Assay Genie is a reliable tool for researchers looking to investigate the role of HSP 70 in health and disease in mouse models.
Product Name:
Mouse HSP-70 (Heat Shock Protein 70) ELISA Kit
SKU:
MOES01127
Target:
Mouse HSP-70 (Heat Shock Protein 70)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP-70/HSPA9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HSP-70/HSPA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HSP-70/HSPA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HSP-70/HSPA9. You can calculate the concentration of Mouse HSP-70/HSPA9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
86-96
96-108
Average (%)
91
91
102
1:4
Range (%)
94-107
85-98
95-111
Average (%)
101
92
102
1:8
Range (%)
97-110
82-97
92-104
Average (%)
103
88
97
1:16
Range (%)
100-112
86-100
92-102
Average (%)
106
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
97-111
102
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
137.2
373.7
1104.6
136.0
354.1
1163.5
Standard deviation
8.8
18.3
53.0
9.0
19.8
37.2
C V (%)
6.41
4.9
4.8
6.62
5.59
3.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse HSP-70/HSPA9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Mouse HSP-70/HSPA9 and analogues was observed.
