The Mouse IL-1α ELISA Kit is a powerful tool tailored to quantitatively measure Interleukin-1 alpha (IL-1α) levels in diverse biological samples with precision and accuracy. IL-1α, an essential pro-inflammatory cytokine, plays a pivotal role in orchestrating various cellular functions, including cell proliferation, differentiation, and apoptosis. Accurate quantification of IL-1α is crucial for unraveling its intricate involvement in immune responses, inflammatory reactions, and its association with diverse inflammatory conditions, autoimmune diseases, and infections. Understanding the levels of IL-1α is fundamental in deciphering its contributions to disease mechanisms and in formulating targeted therapeutic approaches. With exceptional sensitivity and specificity, the Mouse IL-1α ELISA Kit from Assay Genie enables researchers to obtain reliable and reproducible results. Manufactured under stringent quality control measures, this ELISA kit assures robust performance and ease of use, making it an excellent choice for research endeavors focused on exploring the multifaceted roles of IL-1α in immune regulation and inflammatory processes.
Product Name:
Mouse IL-1alpha ELISA Kit
SKU:
AEES00250
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
