The Mouse MUC5B (Mucin 5 Subtype B) ELISA Kit is specifically designed for the precise measurement of mouse MUC5B levels in biological samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.MUC5B is a key mucin protein that plays a vital role in the respiratory system by providing protection and lubrication to the airway epithelium. Dysregulation of MUC5B expression has been associated with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and asthma, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions.
With its reliable performance and ease of use, the Mouse MUC5B ELISA Kit is an indispensable tool for researchers investigating mucin biology and respiratory diseases in preclinical and clinical settings.
Product Name:
Mouse MUC5B (Mucin-5 Subtype B) ELISA Kit
SKU:
MOES01276
Target:
Mouse MUC5B (Mucin-5 Subtype B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC5B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MUC5B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MUC5B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MUC5B. You can calculate the concentration of Mouse MUC5B in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-95
89-103
94-110
Average (%)
90
95
101
1:4
Range (%)
94-106
85-96
87-103
Average (%)
99
90
94
1:8
Range (%)
88-101
82-91
86-99
Average (%)
93
86
91
1:16
Range (%)
89-100
81-96
83-96
Average (%)
95
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.09
8.76
0.99
2.21
8.13
Standard deviation
0.05
0.09
0.39
0.06
0.12
0.43
C V (%)
4.95
4.31
4.45
6.06
5.43
5.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MUC5B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MUC5B in samples. No significant cross-reactivity or interference between Mouse MUC5B and analogues was observed.
