The Mouse NSE (Neuron-Specific Enolase) ELISA Kit is a powerful tool for the precise measurement of NSE levels in mouse serum, plasma, and cell culture supernatants. With outstanding sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research studies.NSE is a key enzyme found in neurons, playing a vital role in energy metabolism and serving as a marker for neuronal damage or injury.
Monitoring NSE levels can provide valuable insights into neurological disorders, brain injuries, and neurodegenerative diseases, making this kit indispensable for researchers in the field of neuroscience.Get your hands on the Mouse NSE ELISA Kit today and unlock new possibilities for your research on brain health and neuronal function.
Product Name:
Mouse NSE (Neuron-Specific Enolase) ELISA Kit
SKU:
MOES00691
Target:
Mouse NSE (Neuron-Specific Enolase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NSE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NSE. You can calculate the concentration of Mouse NSE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
90-102
85-97
Average (%)
103
97
91
1:4
Range (%)
99-112
82-94
93-107
Average (%)
105
87
101
1:8
Range (%)
98-114
82-96
92-105
Average (%)
104
88
99
1:16
Range (%)
99-113
88-101
96-111
Average (%)
106
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
230.4
548.9
2118.2
239.2
496.8
1942.6
Standard deviation
16.1
23.1
74.1
14.8
28.3
79.6
C V (%)
6.99
4.21
3.5
6.19
5.7
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NSE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NSE in samples. No significant cross-reactivity or interference between Mouse NSE and analogues was observed.
