Description
Phospho-BCAR1-Y410 Antibody (CABP1058)
The Phospho-BCAR1-Y410 Antibody (CABP1058) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the phosphorylated Y410 residue of BCAR1, allowing for the detection and analysis of this important protein in various cell types.BCAR1 is known to play a crucial role in cell signaling processes that contribute to cancer progression and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | Phospho-BCAR1-Y410 Antibody |
| SKU: | CABP1058 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | GVYA V | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | CAS, CAS1, CASS1, CRKAS, P130Cas, Phospho-BCAR1-Y410 |
| Positive Sample: | HeLa treated with Pervanadate |
| Cellular Localization: | Cell Junction, Cytoplasm, Focal Adhesion. |
| Calculated MW: | 93kDa |
| Observed MW: | 93kDa |
The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer.
| Purification Method | Affinity purification |
| Gene ID | 9564 |
| RRID | AB_2863930 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (CAB18270). HeLa cells were treated with Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s. |
