The Phospho-CDK1-Y15 Monoclonal Antibody (CABP1350) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal technology, is highly specific to human samples and is validated for use in various applications, including Western blot and immunofluorescence.CDK1, a member of the cyclin-dependent kinase family, plays a crucial role in cell cycle control by regulating the transition from G2 phase to mitosis. Phosphorylation of CDK1 at tyrosine 15 is known to be a key regulatory step in controlling its activity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-CDK1-Y15 Monoclonal Antibody
SKU:
CABP1350
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC55443
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GTYG V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC2, CDC28A, P34CDC2, Phospho-CDK1-Y15
Positive Sample:
HeLa treated with Hydroxyurea
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
983
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using Phospho-CDK1-Y15 Rabbit mAb (CABP1350) at 1:2000 dilution. HeLa cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3 s.
