The Phospho-ErbB2-Y1139 Monoclonal Antibody (CABP1285) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications for the detection and analysis of Phospho-ERBB2 protein in various cell types.Phospho-ERBB2, also known as HER2 or ERBB2, is a receptor tyrosine kinase that plays a critical role in cell signaling pathways associated with cell proliferation and survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-ErbB2-Y1139 Monoclonal Antibody
SKU:
CABP1285
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2723
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PEYV N
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Membrane, Cytoplasm, Nucleus, Nucleus, Single-Pass Type I Membrane Protein, Perinuclear Region.
Calculated MW:
138kDa
Observed MW:
185kDa
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Purification Method
Affinity purification
Gene ID
2064
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-HER2/ErbB2-Y1139 (CABP1285) at 1:500 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
