The Phospho-STAT5-Y694 Antibody (CABP0887) is a high-quality antibody developed for reliable detection and analysis of target proteins. Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. RRID AB_2771572 Gene ID 6776 6777 Swiss Prot Synonym MGF; STAT5; stat5a/stat5b; Phospho-STAT5-Y694
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-STAT5-Y694 Antibody
SKU:
CABP0887
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000-1:10000
IHC-P
1:100 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MGF, STAT5, stat5a/stat5b, Phospho-STAT5-Y694
Positive Sample:
TF-1 treated with hGM-CSF
Cellular Localization:
Cytosol, Nucleoplasm.
Calculated MW:
90kDa
Observed MW:
90 kDa
Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. RRID AB_2771572 Gene ID 6776 6777 Swiss Prot Synonym MGF; STAT5; stat5a/stat5b; Phospho-STAT5-Y694
Purification Method:
Affinity purification
Gene ID:
6776 6777
RRID:
AB_2771572
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from TF-1 cells using Phospho-STAT5-Y694 Rabbit pAb (CABP0887) at 1:10000 dilution incubated at room temperature for 1.5 hours. TF-1 treated with hGM-CSF (10 ng/mL) for 10 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5 s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using Phospho-STAT5-Y694 Rabbit pAb (CABP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-STAT5-Y694 Rabbit pAb (CABP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
