The Phospho-ZAP70-Y493 Antibody (CABP0468) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to the phosphorylated form of ZAP70 at residue Y493 and is validated for use in Western blot applications. By targeting this phosphorylation site, researchers can investigate the activation status of ZAP70 in various cell types, shedding light on its role in immune responses and diseases such as cancer and autoimmune disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-ZAP70-Y493 Antibody
SKU:
CABP0468
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SYYT A
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:100
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7535
RRID
AB_2771651
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-ZAP70-Y493 Rabbit pAb (CABP0468) at 1:1000 dilution. Jurkat cells were treated with Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight or treated with Pervanadate (1 mM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Phospho-ZAP70-Y493 Rabbit pAb (CABP0468). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
