The QuickStep Mouse IgA (Immunoglobulin A) ELISA Kit is a powerful tool for the precise measurement of IgA levels in mouse serum, plasma, and cell culture supernatants. With its excellent sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research applications.Immunoglobulin A plays a critical role in the immune system, protecting mucosal surfaces from pathogens and foreign substances. Dysregulation of IgA levels has been linked to various autoimmune diseases, inflammatory conditions, and infections, highlighting the importance of studying IgA dynamics. The QuickStep Mouse IgA ELISA kit provides researchers with a reliable means of quantifying IgA levels and investigating its role in disease development and progression.Whether you are studying immune responses, investigating disease mechanisms, or developing therapeutic strategies, the QuickStep Mouse IgA ELISA Kit offers a versatile and efficient solution for your research needs. Trust in its precision, sensitivity, and ease of use to accelerate your discoveries in the field of immunology.
Product Name:
Mouse IgA (Immunoglobulin A) Quickstep ELISA Kit
SKU:
QSES038
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
