The QuickStep Mouse IgG3 (Immunoglobulin G3) ELISA Kit is a premier assay designed for the precise and quantitative detection of IgG3 levels in mouse biological samples. IgG3 is an essential subtype of immunoglobulin G that plays a crucial role in the immune system, participating in immune responses against pathogens and infections. This ELISA kit offers advanced technology and high sensitivity to accurately measure the levels of IgG3, providing valuable insights into the immune system's function and response in mouse models. Researchers can rely on the QuickStep Mouse IgG3 ELISA Kit to deliver accurate and reproducible results, enabling in-depth analysis of the immune system dynamics and IgG3-mediated immune processes. The kit's exceptional sensitivity and specificity ensure reliable measurements, making it a valuable tool for immunology research, disease studies, and therapeutic development. Manufactured under strict quality control standards, the QuickStep Mouse IgG3 ELISA Kit guarantees robust performance and ease of use, making it an excellent choice for researchers seeking high-quality data for their studies. Unlock new perspectives on immune responses and immunoglobulin dynamics in mouse models with this cutting-edge ELISA kit.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
