The Rat ADH (Antidiuretic Hormone) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Antidiuretic Hormone in various biological samples. Antidiuretic Hormone, also known as vasopressin, plays a crucial role in regulating water balance, blood pressure, and concentration of urine.
Accurate measurement of Antidiuretic Hormone is essential for understanding its physiological functions and its role in various conditions related to water balance and renal function. The Rat ADH ELISA Kit provides exceptional sensitivity and specificity for precise and reliable results. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an invaluable tool for researchers studying hormone regulation and related mechanisms.
Product Name:
Rat ADH (Antidiuretic Hormone) ELISA Kit
SKU:
AEES00442
Target:
Rat ADH (Antidiuretic Hormone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ADH. During the reaction, ADH in the sample or standard competes with a fixed amount of ADH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ADH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ADH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
99-116
86-98
Average (%)
95
106
91
1:4
Range (%)
89-105
92-106
98-112
Average (%)
96
99
106
1:8
Range (%)
87-99
86-99
95-109
Average (%)
94
92
101
1:16
Range (%)
83-96
94-107
99-115
Average (%)
89
100
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
102
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
92-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.2
152.8
417.9
50.9
157.9
442.6
Standard deviation
2.8
6.4
15.9
2.9
7.1
18.6
C V (%)
5.69
4.19
3.8
5.7
4.5
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ADH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ADH in samples. No significant cross-reactivity or interference between ADH and analogues was observed.
