Isocitrate dehydrogenases catalyses the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The enzymes are divided into two categories: those that use NAD(+) as an electron acceptor and those that utilize NADP(+). Three NAD(+)-dependent isocitrate dehydrogenases that localise to the mitochondrial matrix have been identified. In addition, two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic have been identified. Each isozyme that is dependent on NADP(+) is a homodimer. This gene encodes the NADP(+)-dependent isocitrate dehydrogenase, which is present in both the cytoplasm and peroxisomes. Glioma susceptibility 1 and Metaphyseal enchondromatosis with D-2-hydroxyglutaric aciduria are two diseases assocaited with IDH1. The HIV life cycle and amino acid metabolism are two pathways related to Isocitrate dehydrogenases. Human IDH1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Recombinant Human Isocitrate Dehydrogenase [NADP] Cytoplasmic is produced by our Mammalian expression system and the target gene encoding Met1-Leu414 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
47.7 kDa
AP Mol Mass:
45 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
