Description
Sheep Cytokine Array (10 targets) (SARB0093)
The Sheep Cytokine Array, capable of detecting 10 different targets, is a powerful tool for researchers studying cytokine expression profiles. This array allows for the simultaneous detection of multiple cytokines in a single sample, providing valuable insight into immune responses, inflammation, and disease progression.With a focus on cytokines produced by sheep, this array offers a comprehensive view of the immune response specific to this species. The targets included in this array cover a range of cytokines involved in various physiological processes, making it a versatile tool for a wide range of research applications.Using this Sheep Cytokine Array, researchers can uncover important information about the role of cytokines in immune regulation, infectious diseases, and other complex biological processes.
By analyzing the expression levels of multiple cytokines simultaneously, researchers can gain a deeper understanding of the intricate mechanisms underlying these processes.Overall, the Sheep Cytokine Array is a valuable resource for researchers looking to explore the complex interplay of cytokines in sheep physiology and pathology. Its high sensitivity and specificity make it a reliable tool for generating meaningful data and advancing our understanding of immune responses in sheep.
| Product Sku: | SARB0093 |
| Size: | 2, 4, or 8 |
| Species Detected: | Sheep |
| Number of Targets Detected: | 10 |
| Gene Symbols: | CXCL8, DCN, FRZB, IFNG, IL17A, IL21, IP-10, TNF, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Decorin
| IFN-gamma
| IL-17A
| IL-21
| IL-8 (CXCL8)
|
IP-10 (CXCL10)
| MIG (CXCL9)
| sFRP-3
| TNF alpha
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Sheep Cytokine Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
