Anti-ZP3 Antibody (CAB8355)

Antibody Name:	Anti-ZP3 Antibody
Antibody SKU:	CAB8355
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-390 of human ZP3 (NP_001103824.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-390 of human ZP3 (NP_001103824.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LTFS LRLM EENW NAEK RSPT FHLG DAAH LQAE IHTG SHVP LRLF VDHC VATP TPDQ NASP YHTI VDFH GCLV DGLT DASS AFKV PRPG PDTL QFTV DVFH FAND SRNM IYIT CHLK VTLA EQDP DELN KACS FSKP SNSW FPVE GSAD ICQC CNKG DCGT PSHS RRQP HVMS QWSR SASR NRRH VTEE ADVT VGPL IFLD RRGD HEVE QWAL PSDT SVVL L
Gene ID:	7784
Uniprot:	P21754
Cellular Location:	Cell membrane, Secreted, Single-pass type I membrane protein, extracellular matrix, extracellular space
Calculated MW:	41kDa/47kDa
Observed MW:	47kDa

Synonyms:

ZP3, ZP3A, ZP3B, ZPC, Zp-3

Background:

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.

UniProt Protein Function:	ZP3: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. Belongs to the ZP domain family. ZPC subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Extracellular matrix Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: acrosome; cytoplasm; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; multivesicular body; perinuclear region of cytoplasm; plasma membrane; secretory granule Molecular Function:acrosin binding; carbohydrate binding; manganese ion transmembrane transporter activity; protein binding; signal transducer activity; store-operated calcium channel activity Biological Process: binding of sperm to zona pellucida; blastocyst formation; humoral immune response mediated by circulating immunoglobulin; intracellular protein transport; manganese ion transport; negative regulation of transcription, DNA-dependent; oocyte development; phosphoinositide-mediated signaling; positive regulation of humoral immune response; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-4 production; positive regulation of leukocyte migration; positive regulation of phosphatidylinositol biosynthetic process; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of type IV hypersensitivity
NCBI Summary:	The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P21754
NCBI GenInfo Identifier:	251757420
NCBI Gene ID:	7784
NCBI Accession:	P21754.2
UniProt Secondary Accession:	P21754,Q06633, Q29RW0,
UniProt Related Accession:	P21754
Molecular Weight:	41,272 Da
NCBI Full Name:	Zona pellucida sperm-binding protein 3
NCBI Synonym Full Names:	zona pellucida glycoprotein 3 (sperm receptor)
NCBI Official Symbol:	ZP3
NCBI Official Synonym Symbols:	ZPC; ZP3A; ZP3B; Zp-3
NCBI Protein Information:	zona pellucida sperm-binding protein 3
UniProt Protein Name:	Zona pellucida sperm-binding protein 3
UniProt Synonym Protein Names:	Sperm receptor; ZP3A/ZP3B; Zona pellucida glycoprotein 3; Zp-3
Protein Family:	Zona pellucida sperm-binding protein
UniProt Gene Name:	ZP3
UniProt Entry Name:	ZP3_HUMAN

	Western blot analysis of extracts of SKOV3 cells, using ZP3 antibody (CAB8355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody (CAB8355) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody (CAB8355) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-ZP3 Antibody (CAB13156)	Human ZP3 / Zona pellucida sperm-binding protein 3 ELISA Kit
	Human Zona pellucida sperm-binding protein 3 (ZP3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)