The SUPT5H/SPT5 Monoclonal Antibody (CAB19225) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables enzyme binding activity and protein heterodimerization activity. Involved in positive regulation of macroautophagy; regulation of RNA metabolic process; and transcription elongation from RNA polymerase II promoter. Located in nucleoplasm. Part of DSIF complex.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SUPT5H/SPT5 Monoclonal Antibody
SKU:
CAB19225
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2380
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPT5, SPT5H, Tat-CT1, SUPT5H/SPT5
Positive Sample:
HeLa,
Cellular Localization:
Nucleus.
Calculated MW:
121kDa
Observed MW:
150kDa/
Enables enzyme binding activity and protein heterodimerization activity. Involved in positive regulation of macroautophagy; regulation of RNA metabolic process; and transcription elongation from RNA polymerase II promoter. Located in nucleoplasm. Part of DSIF complex.
Purification Method
Affinity purification
Gene ID
6829
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using SUPT5H/SPT5 Rabbit mAb (CAB19225) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using SUPT5H/SPT5 Rabbit mAb (CAB19225) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells using SUPT5H/SPT5 Rabbit mAb (CAB19225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using SUPT5H/SPT5 Rabbit mAb (CAB19225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation of SUPT5H/SPT5 from 300 µg extracts of HeLa cells was performed using 3 µg of SUPT5H/SPT5 Rabbit mAb (CAB19225). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using SUPT5H/SPT5 Rabbit mAb (CAB19225) at a dilution of 1:1000.
