Description
Canine Inflammation Array (20 targets) (SARB0010)
The Canine Inflammation Array (SARB0010) is a comprehensive research tool designed for studying inflammatory responses in dogs. This array allows for the simultaneous detection of 20 different inflammation-related protein targets in canine samples, providing valuable insights into the immune response in these animals.With a focus on key inflammatory markers, the Canine Inflammation Array offers a high level of sensitivity and specificity, making it an ideal choice for researchers studying conditions such as arthritis, allergies, and inflammatory bowel disease in dogs. By analyzing the expression levels of multiple inflammatory markers at once, this array enables a more comprehensive understanding of the inflammatory processes occurring in canine diseases.
Whether investigating the efficacy of anti-inflammatory treatments or exploring the molecular mechanisms underlying inflammation in dogs, the Canine Inflammation Array provides a powerful tool for advancing research in veterinary medicine. With its user-friendly protocol and reliable results, this array is sure to facilitate important discoveries in the field of canine inflammation research.
| Product Sku: | SARB0010 |
| Size: | 2, 4, or 8 |
| Species Detected: | Canine |
| Number of Targets Detected: | 20 |
| Gene Symbols: | CCL2, CCL4, CSF2, CXCL8, IFNG, IL10, IL12B, IL17A, IL1B, IL6|TNF, TNFRSF1A |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1) | IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3) |
IL-10
| IL-12 p40
| IL-13 | IL-17A
| IL-21 |
IL-6
| IL-8 (CXCL8)
| MCP-1 (CCL2)
| MIP-1 beta (CCL4)
| RAGE
|
| RANTES (CCL5) | Resistin
| TNF alpha
| TNF RI (TNFRSF1A)
| Trappin-2
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Canine Inflammation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
