The Human Beta-EP (Beta-Endorphin) ELISA Kit is a reliable and sensitive tool for accurately measuring beta-endorphin levels in human biological samples. This kit is designed for use with serum, plasma, and cell culture supernatants, providing researchers with consistent and reproducible results.Beta-endorphin is a key peptide hormone known for its role in regulating pain perception, stress response, and mood. Dysregulation of beta-endorphin levels has been linked to various disorders such as chronic pain, mood disorders, and addictive behaviors.
By accurately measuring beta-endorphin levels, researchers can gain valuable insights into these conditions and potentially develop targeted therapies.With its high sensitivity and specificity, the Human Beta-EP ELISA Kit is an essential tool for studying the physiological and pathological roles of beta-endorphin in human health and disease. Explore the potential of beta-endorphin research with this advanced ELISA kit from Assay Genie.
Product Name:
Human beta-EP (Beta-Endorphin) ELISA Kit
SKU:
HUES01751
Target:
Human beta-EP (Beta-Endorphin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-EP. During the reaction, β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
89-103
96-113
Average (%)
95
94
104
1:4
Range (%)
84-95
94-109
101-115
Average (%)
90
100
106
1:8
Range (%)
85-96
90-105
94-109
Average (%)
91
96
101
1:16
Range (%)
85-100
89-105
99-116
Average (%)
92
96
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
97
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.5
153.0
435.3
46.7
158.1
426.0
Standard deviation
2.9
7.5
20.0
2.8
6.6
13.6
C V (%)
6.37
4.9
4.59
6.0
4.17
3.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of β-EP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.
