The Human FGF9 (Fibroblast Growth Factor 9) CLIA Kit is a powerful tool for the precise measurement of FGF9 levels in human samples including serum, plasma, and cell culture supernatants. This CLIA kit is known for its high sensitivity and specificity, providing researchers with accurate and consistent results for a variety of research purposes.FGF9 is a critical growth factor involved in various cellular processes such as cell proliferation, differentiation, and survival. It plays a crucial role in multiple biological functions, making it a key molecule in the study of developmental biology, cancer research, and tissue regeneration.
The Human FGF9 CLIA Kit allows researchers to quantify FGF9 levels in biological samples, aiding in the understanding of its role in disease progression and potential therapeutic interventions.Overall, the Human FGF9 CLIA Kit from Assay Genie is a valuable tool for researchers looking to study the role of FGF9 in human health and disease. Its high sensitivity and specificity make it a reliable choice for accurate quantification of FGF9 levels in various biological samples.
Product Name:
Human FGF9 (Fibroblast Growth Factor 9) CLIA Kit
SKU:
HUES00656
Target:
Human FGF9 (Fibroblast Growth Factor 9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGF9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGF9. You can calculate the concentration of Human FGF9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-118
91-105
90-107
Average (%)
110
96
97
1:4
Range (%)
94-112
99-114
89-101
Average (%)
102
105
94
1:8
Range (%)
103-117
96-112
90-105
Average (%)
109
102
96
1:16
Range (%)
84-97
102-119
102-117
Average (%)
91
109
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
106
EDTA plasma (n=5)
98-110
103
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.57
135.24
447.28
52.74
139.87
490.99
Standard deviation
6.29
14.82
31.58
4.74
11.94
41.69
C V (%)
12.95
10.96
7.06
8.99
8.54
8.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGF9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF9 in samples. No significant cross-reactivity or interference between Human FGF9 and analogues was observed.
