Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
< 1.0 EU/mg of the protein as determined by the LAL method
Purity:
> 90% as determined by reducing SDS-PAGE.
Calculated MW:
30.6 kDa
Observed MW:
35 kDa
Bio-Activity:
Not validated for activity
Form:
Lyophilized powder
Source:
HEK293 Cells-derived Human IL-1α protein Met1-Ala271, with an C-terminal His
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Storage:
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 ℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
