Description
Human Inflammation Array (20 targets) (SARB0051)
The Human Inflammation Array - 20 Targets (SARB0051) is a comprehensive tool for studying inflammation and immune responses in human samples. This array allows for the simultaneous detection of 20 inflammation-related proteins, providing valuable insights into the complex pathways involved in inflammatory processes.With a high level of sensitivity and specificity, this array is ideal for researchers studying various conditions such as autoimmune disorders, infectious diseases, and inflammatory conditions.
The array enables the profiling of multiple targets in a single sample, saving time and resources while providing a comprehensive view of the inflammatory status.Whether you are exploring the role of cytokines, chemokines, or growth factors in inflammation, the Human Inflammation Array - 20 Targets offers a powerful tool for advancing your research. Trust Assay Genie for reliable and accurate results in your studies of inflammation and immune response.
| Product Sku: | SARB0051 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 20 |
| Gene Symbols: | CCL15, CCL2, CCL3, CCL4, CCL5, CCL8, CSF1, CXCL10, CXCL9, ICAM1|IL15, IL16, IL17A, IL6R, LTA, PDGFB, TGFB1, TNF, TNFRSF1A, TNFRSF1B |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | MAPK Signaling / NFkB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
ICAM-1 (CD54)
| IL-6 R
| IL-15
| IL-16
| IL-17A
|
IP-10 (CXCL10)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| M-CSF
| MIG (CXCL9)
|
MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| RANTES (CCL5)
| TGF beta 1
|
TNF alpha
| TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| PDGF-BB
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Inflammation Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
