Description
system_update_altDatasheet
Human Inflammation Array (40 targets) (SARB0052)
The Human Inflammation Array with 40 Targets (SARB0052) from Assay Genie is a comprehensive tool for studying inflammatory responses in human samples. This array allows for the simultaneous detection of 40 inflammation-related proteins, providing researchers with a global view of the inflammatory profile in their samples.With a focus on key targets involved in inflammation, such as cytokines, chemokines, and growth factors, this array enables high-throughput analysis of immune responses in a variety of experimental settings. The high sensitivity and specificity of the assay ensure reliable and reproducible results, making it a valuable tool for research in immunology, oncology, and inflammatory diseases.
Whether investigating the inflammatory status of cancer cells, studying the effects of drugs on immune response, or exploring potential biomarkers for inflammatory conditions, the Human Inflammation Array with 40 Targets offers a comprehensive solution for researchers seeking to understand the complex interplay of inflammatory mediators in human samples.
| Product Sku: | SARB0052 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 40 |
| Gene Symbols: | CCL1, CCL11, CCL15, CCL2, CCL24, CCL3, CCL4, CCL5, CCL8, CSF1|CSF2, CSF3, CXCL10, CXCL8, CXCL9, ICAM1, IFNG, IL10, IL11, IL12A, IL12B, IL13, IL15, IL16, IL17A, IL1A, IL1B, IL2, IL3, IL4, IL6, IL6R, IL7, LTA, PDGFB, TGFB1, TIMP2, TNF, TNFRSF1A, TNFRSF1B |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| GCSF
| GM-CSF
| ICAM-1 (CD54)
|
IFN-gamma
| I-309 (TCA-3/CCL1)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
|
IL-3
| IL-4
| IL-6
| IL-6 R
| IL-7
|
IL-8 (CXCL8)
| IL-10
| IL-11
| IL-12 p40
| IL-12 p70
|
IL-13
| IL-15
| IL-16
| IL-17A
| IP-10 (CXCL10)
|
MCP-1 (CCL2)
| MCP-2 (CCL8)
| M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| RANTES (CCL5)
| TGF beta 1
| TNF alpha
|
TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| PDGF-BB
| TIMP-2
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Inflammation Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
