Human RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit
The Human RANKL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit is a cutting-edge assay designed for the precise measurement of RANKL levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.RANKL is a critical signaling protein involved in bone metabolism and immune system regulation. It plays a key role in osteoclast differentiation and activation, as well as in inflammatory responses.
Dysregulation of RANKL has been linked to osteoporosis, arthritis, and other bone-related disorders, highlighting its importance as a biomarker for disease diagnosis and monitoring.With the Human RANKL CLIA Kit, researchers can gain valuable insights into RANKL activity and its impact on various physiological processes. This advanced assay is an invaluable tool for studying bone health, immune function, and potential therapeutic interventions targeting RANKL signaling pathways.
Product Name:
Human RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit
SKU:
HUES00796
Target:
Human RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RANκL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANκL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RANκL. You can calculate the concentration of Human RANκL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
104-115
92-105
Average (%)
96
109
98
1:4
Range (%)
97-109
98-115
88-101
Average (%)
103
105
94
1:8
Range (%)
91-105
91-106
100-115
Average (%)
98
97
107
1:16
Range (%)
89-104
96-111
100-114
Average (%)
95
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.75
176.51
997.84
104.15
184.36
971.82
Standard deviation
11.43
16.15
89.61
11.93
13.79
111.08
C V (%)
10.61
9.15
8.98
11.45
7.48
11.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RANκL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RANκL in samples. No significant cross-reactivity or interference between Human RANκL and analogues was observed.
