Human TNF- alpha (Tumor Necrosis Factor Alpha) ELISA Kit
The Human TNF-alpha (Tumor Necrosis Factor-alpha) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of TNF-alpha levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.TNF-alpha is a key cytokine involved in inflammation, immune response, and apoptosis. Dysregulation of TNF-alpha has been associated with various inflammatory diseases, autoimmune disorders, and cancer.
By accurately measuring TNF-alpha levels, researchers can gain valuable insights into the pathogenesis of these diseases and develop potential therapeutic strategies.The Human TNF-alpha ELISA Kit from Assay Genie offers researchers a reliable method for detecting and quantifying TNF-alpha levels in human samples, providing a valuable tool for studying the role of TNF-alpha in disease pathophysiology and identifying potential therapeutic targets.
Product Name:
Human TNF- alpha (Tumor Necrosis Factor Alpha) ELISA Kit
SKU:
HUES01395
Target:
Human TNF- alpha (Tumor Necrosis Factor Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
84-97
88-99
Average (%)
99
91
93
1:4
Range (%)
99-116
87-99
91-104
Average (%)
107
93
98
1:8
Range (%)
97-113
81-93
92-109
Average (%)
104
88
99
1:16
Range (%)
96-112
85-98
95-107
Average (%)
103
90
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.1
79.5
183.0
22.7
86.5
177.4
Standard deviation
1.5
3.8
9.7
1.2
4.0
7.6
C V (%)
6.22
4.78
5.3
5.29
4.62
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.
