The Mouse IL-17 ELISA Kit (MOES00666) is a highly sensitive and specific assay designed for accurate detection of Interleukin-17 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Interleukin-17 is a key cytokine involved in inflammatory responses and immune regulation. It plays a critical role in autoimmune diseases, inflammation, and host defense against infections.
Monitoring IL-17 levels is important for understanding the immune response and developing therapeutic strategies for related conditions.With its high sensitivity and specificity, the Mouse IL-17 ELISA Kit (MOES00666) is a valuable tool for researchers studying the role of IL-17 in various disease models and exploring potential treatments targeting this cytokine.
Product Name:
Mouse IL-17 ELISA Kit
SKU:
MOES00666
Target:
Mouse IL-17
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-17A. You can calculate the concentration of Mouse IL-17A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
90-108
98-112
Average (%)
92
98
105
1:4
Range (%)
95-110
85-97
91-108
Average (%)
103
90
98
1:8
Range (%)
98-114
81-92
91-104
Average (%)
105
87
98
1:16
Range (%)
98-111
87-99
96-109
Average (%)
105
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
102
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.5
108.6
431.3
46.1
109.5
437.9
Standard deviation
2.8
5.4
22.9
2.3
5.0
16.2
C V (%)
5.89
4.97
5.31
4.99
4.57
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-17A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-17A in samples. No significant cross-reactivity or interference between Mouse IL-17A and analogues was observed.
