Rat PDGF-BB (Platelet-Derived Growth Factor-BB) CLIA Kit
The Rat PDGF-BB (Platelet-Derived Growth Factor-BB) CLIA Kit is a state-of-the-art assay designed for the accurate quantification of PDGF-BB levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a wide range of research applications.PDGF-BB is a critical growth factor that plays a key role in various biological processes, including cell growth, proliferation, and wound healing.
Dysregulation of PDGF-BB is associated with conditions such as cancer, fibrosis, and vascular diseases, making it a valuable biomarker for studying disease pathogenesis and developing targeted therapies.With its advanced technology and ease of use, the Rat PDGF-BB CLIA Kit from AssayGenie is an essential tool for researchers investigating the role of PDGF-BB in health and disease. Trust in AssayGenie for high-quality and reliable research tools to advance your scientific discoveries.
Product Name:
Rat PDGF-BB (Platelet-Derived Growth Factor-BB) CLIA Kit
SKU:
RTES00322
Target:
Rat PDGF-BB (Platelet-Derived Growth Factor-BB)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-BB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-BB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PDGF-BB. You can calculate the concentration of Rat PDGF-BB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
96-114
88-100
Average (%)
100
104
95
1:4
Range (%)
100-118
100-112
95-111
Average (%)
108
106
101
1:8
Range (%)
86-101
96-106
89-106
Average (%)
92
101
97
1:16
Range (%)
91-106
85-97
96-108
Average (%)
97
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
99-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.86
128.06
267.17
41.28
132.14
252.93
Standard deviation
4.57
12.75
17.71
3.33
15.79
21.27
C V (%)
10.42
9.96
6.63
8.07
11.95
8.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PDGF-BB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PDGF-BB in samples. No significant cross-reactivity or interference between Rat PDGF-BB and analogues was observed.
