The MRPL28 Monoclonal Antibody (CAB0452) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and has been validated for use in various applications, including Western blotting.MRPL28 is essential for maintaining mitochondrial function and mutations in this gene have been linked to mitochondrial disorders and diseases.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MRPL28 Monoclonal Antibody
SKU:
CAB0452
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2507
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p15, MAAT1, MRPL28
Positive Sample:
HeLa, 293T, K-562, Mouse kidney, Mouse heart, Rat kidney, Rat heart, Rat pancreas
Cellular Localization:
Mitochondrion.
Calculated MW:
30kDa
Observed MW:
30kDa/30kd
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.
Purification Method
Affinity purification
Gene ID
10573
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MRPL28 Rabbit mAb (CAB0452) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using MRPL28 Rabbit mAb (CAB0452) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation of MRPL28 from 300 µg extracts of K-562 cells was performed using 2 µg of MRPL28 Rabbit mAb (CAB0452). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using MRPL28 Rabbit mAb (CAB0452) at a dilution of 1:2000.
